000 04418nam a22005295i 4500
001 978-3-642-40190-9
003 DE-He213
005 20140220082918.0
007 cr nn 008mamaa
008 131016s2013 gw | s |||| 0|eng d
020 _a9783642401909
_9978-3-642-40190-9
024 7 _a10.1007/978-3-642-40190-9
_2doi
050 4 _aQD450-882
072 7 _aPNR
_2bicssc
072 7 _aSCI013050
_2bisacsh
082 0 4 _a541
_223
100 1 _aBrøndsted Nielsen, Steen.
_eeditor.
245 1 0 _aPhotophysics of Ionic Biochromophores
_h[electronic resource] /
_cedited by Steen Brøndsted Nielsen, Jean Ann Wyer.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2013.
300 _aXIII, 230 p. 125 illus., 102 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aPhysical Chemistry in Action,
_x2197-4349
505 0 _aConcepts -- Introduction and new aspects -- Experimental techniques -- Theoretical Methods -- Photo-initiated Dynamics and Spectroscopy of the Deprotonated Green Fluorescent Protein Chromophore -- Fluorescence from Gas-phase Biomolecular Ions -- Spectroscopy of Ferric Heme and Protoporphyrin IX Ions in vacuo -- UV-Visible Absorption Spectroscopy of Protein Ions -- Excited-state Dynamics of Protonated Aromatic Amino Acids -- UV Photophysics of DNA and RNA Nucleotides in vacuo: Dissociation Channels, Time Scales, and Electronic Spectra -- Action Spectroscopy of Gas-phase Peptide Ions with Energetic Photons.
520 _aThis book provides a concise overview of the photophysics and spectroscopy of biochromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states. This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ion spectroscopy.
650 0 _aChemistry.
650 0 _aSpectroscopy.
650 0 _aBioorganic chemistry.
650 0 _aChemistry, Physical organic.
650 0 _aBiochemistry.
650 1 4 _aChemistry.
650 2 4 _aPhysical Chemistry.
650 2 4 _aBiophysics and Biological Physics.
650 2 4 _aSpectroscopy/Spectrometry.
650 2 4 _aBioorganic Chemistry.
650 2 4 _aBiochemistry, general.
700 1 _aWyer, Jean Ann.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783642401893
830 0 _aPhysical Chemistry in Action,
_x2197-4349
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-642-40190-9
912 _aZDB-2-CMS
999 _c98566
_d98566