000 03342nam a22004815i 4500
001 978-3-319-00336-8
003 DE-He213
005 20140220082838.0
007 cr nn 008mamaa
008 130511s2013 gw | s |||| 0|eng d
020 _a9783319003368
_9978-3-319-00336-8
024 7 _a10.1007/978-3-319-00336-8
_2doi
050 4 _aQD380-388
072 7 _aPNNP
_2bicssc
072 7 _aTEC055000
_2bisacsh
082 0 4 _a541.2254
_223
100 1 _aYanan, Yue.
_eauthor.
245 1 0 _aHow Free Cationic Polymer Chains Promote Gene Transfection
_h[electronic resource] /
_cby Yue Yanan.
264 1 _aHeidelberg :
_bSpringer International Publishing :
_bImprint: Springer,
_c2013.
300 _aXVIII, 94 p. 45 illus., 38 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
505 0 _aIntroduction and background -- Revisiting complexation between DNA and polyethylenimine: the effect of uncomplexed chains free in the solution mixture on gene transfection -- Revisiting complexation between DNA and polyethylenimine: the effect of length of free polycationic chains on gene transfection -- Quantitative comparison of endocytosis and intracellular trafficking of DNA/polymer complexes in the absence/presence of free polycationic chains.
520 _aIn this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.
650 0 _aChemistry.
650 0 _aChemistry, Organic.
650 0 _aPolymers.
650 0 _aNucleic acids.
650 1 4 _aChemistry.
650 2 4 _aPolymer Sciences.
650 2 4 _aOrganic Chemistry.
650 2 4 _aNucleic Acid Chemistry.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783319003351
830 0 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-319-00336-8
912 _aZDB-2-CMS
999 _c96399
_d96399