000			05378nam a22004935i 4500
001			978-1-4614-6651-2
003			DE-He213
005			20140220082825.0
007			cr nn 008mamaa
008			130527s2013 xxu| s |||| 0|eng d
020			_a9781461466512 _9978-1-4614-6651-2
024	7		_a10.1007/978-1-4614-6651-2 _2doi
050		4	_aRG133.5
072		7	_aMFKC _2bicssc
072		7	_aHEA039060 _2bisacsh
082	0	4	_a612.6 _223
100	1		_aGardner, David K. _eeditor.
245	1	0	_aHuman Gametes and Preimplantation Embryos _h[electronic resource] : _bAssessment and Diagnosis / _cedited by David K. Gardner, Denny Sakkas, Emre Seli, Dagan Wells.
264		1	_aNew York, NY : _bSpringer New York : _bImprint: Springer, _c2013.
300			_aXV, 306 p. 64 illus., 48 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aCurrent Practices and Regulations for Embryo Transfer Worldwide: Implications on IVF Outcome -- Analysis of the Zona Pellucida as an Indicator of Oocyte Developmental Potential -- Morphological Assessment of Oocytes, Pronuclear and Cleavage Stage Embryos -- Morphological Assessment of Blastocyst Stage Embryos: Types of Grading Systems and Their Reported Outcomes -- Real Time Imaging Strategies to Improve Morphological Assessment -- Limitations and Benefits of Morphologic Embryo Assessment Strategies: How Far Can Morphological Assessment Go in the Identification of Viable Embryos? -- Sperm Morphologic Characteristics and Their Impact on Embryo Quality and Pregnancy Outcome -- Objective Biomarkers of Sperm Development and Fertility: Assessment of Sperm-Zona Pellucida Binding Ability and Hyaluronic Acid Mediated Selection of Sperm for ICSI Fertilization -- Selecting the Best Sperm and Its Implications in Clinical Practice -- The Origins of Aneuploidy in Human Embryos -- Preimplantation Genetic Screening and Diagnosis Using Fluorescent In Situ Hybridization (FISH) -- Single Nucleotide Polymorphisms and Next Generation Sequencing -- Use of Comparative Genomic Hybridization (CGH) and Microarray-CGH for Preimplantation Genetic Screening -- Quantitative SNP Array and Real-Time PCR Based Human Preimplantation Embryo Aneuploidy Screening -- Regulation of Gene Expression in the Oocyte and Early Embryo: Implications for Transcriptomic and Proteomic Embryo Assessment -- Transcriptomics Technology: Promise and Potential Pitfalls -- Transcriptomic Analysis of Cumulus and Granulosa Cells as a Marker of Embryo Viability -- Mass Spectrometry, Proteomics and the Study of Sperm Cell Biology -- Proteomic Analysis of Embryo Viability -- Metabolism of the Viable Human Embryo -- Analysis of Respiration as an Indicator of Oocyte and Embryo Developmental Potential -- Mitochondrial Activity as a Biomarker of Gamete and Embryo Health -- Carbohydrate Analysis and Embryo Viability -- Identification of Viable Embryos by Non-invasive Measurement of Amino Acids in Culture Media -- Metabolomic Profiling of Embryos Using Spectroscopy -- Microfluidic Devices for the Analysis of Gamete and Embryo Physiology.
520			_aIn recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates. Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of “omics” approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine.
650		0	_aMedicine.
650		0	_aGynecology.
650		0	_aObstetrics.
650		0	_aReproductive Medicine.
650	1	4	_aMedicine & Public Health.
650	2	4	_aReproductive Medicine.
650	2	4	_aObstetrics/Perinatology.
650	2	4	_aGynecology.
700	1		_aSakkas, Denny. _eeditor.
700	1		_aSeli, Emre. _eeditor.
700	1		_aWells, Dagan. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781461466505
856	4	0	_uhttp://dx.doi.org/10.1007/978-1-4614-6651-2
912			_aZDB-2-SME
999			_c95719 _d95719