000 03194nam a22004815i 4500
001 978-3-642-42008-5
003 DE-He213
005 20140220082522.0
007 cr nn 008mamaa
008 131202s2014 gw | s |||| 0|eng d
020 _a9783642420085
_9978-3-642-42008-5
024 7 _a10.1007/978-3-642-42008-5
_2doi
050 4 _aQD71-142
072 7 _aPNFC
_2bicssc
072 7 _aSCI013010
_2bisacsh
082 0 4 _a543.8
_223
100 1 _aWang, Fangjun.
_eauthor.
245 1 0 _aApplications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis
_h[electronic resource] /
_cby Fangjun Wang.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2014.
300 _aXIII, 94 p. 42 illus., 23 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
505 0 _aIntroduction -- Online sample injection and multidimensional chromatography separation by using strong cation exchange monolithic column -- Development of polymer-based hydrophobic monolithic columns and their applications in proteome analysis -- Large-scale proteome and phosphoproteome quantification by using dimethylation isotope labeling.
520 _aIn this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity, and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.
650 0 _aChemistry.
650 0 _aMass spectrometry.
650 0 _aChromatography.
650 0 _aProteomics.
650 1 4 _aChemistry.
650 2 4 _aChromatography.
650 2 4 _aProteomics.
650 2 4 _aMass Spectrometry.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783642420078
830 0 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-642-42008-5
912 _aZDB-2-CMS
999 _c93513
_d93513