000			03007nam a22005895i 4500
001			978-90-481-3132-7
003			DE-He213
005			20140220084557.0
007			cr nn 008mamaa
008			100301s2010 ne | s |||| 0|eng d
020			_a9789048131327 _9978-90-481-3132-7
024	7		_a10.1007/978-90-481-3132-7 _2doi
050		4	_aQH324
072		7	_aPS _2bicssc
072		7	_aPDN _2bicssc
072		7	_aSCI008000 _2bisacsh
072		7	_aSCI043000 _2bisacsh
082	0	4	_a570.28 _223
100	1		_aPestana, Erika. _eauthor.
245	1	0	_aEarly, rapid and sensitive veterinary molecular diagnostics - real time PCR applications _h[electronic resource] / _cby Erika Pestana, Sandor Belak, Adama Diallo, John R. Crowther, Gerrit J. Viljoen.
264		1	_aDordrecht : _bSpringer Netherlands, _c2010.
300			_aXIV, 310p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aBackground -- Traditional PCR -- Real-Time PCR – The Basic Principles -- New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases -- Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens -- PCR Laboratory Set-up -- Analysis and Troubleshooting -- Specifications for PCR Machines.
520			_aThis book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.
650		0	_aLife sciences.
650		0	_aMedicine.
650		0	_aMedical virology.
650		0	_aEmerging infectious diseases.
650		0	_aAgriculture.
650		0	_aCytology _xResearch_xMethodology.
650	1	4	_aLife Sciences.
650	2	4	_aBiological Techniques.
650	2	4	_aInfectious Diseases.
650	2	4	_aVirology.
650	2	4	_aMolecular Medicine.
650	2	4	_aAgriculture.
650	2	4	_aBiomedicine general.
700	1		_aBelak, Sandor. _eauthor.
700	1		_aDiallo, Adama. _eauthor.
700	1		_aCrowther, John R. _eauthor.
700	1		_aViljoen, Gerrit J. _eauthor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9789048131310
856	4	0	_uhttp://dx.doi.org/10.1007/978-90-481-3132-7
912			_aZDB-2-SBL
999			_c113273 _d113273