000			02894nam a22005175i 4500
001			978-1-4419-1304-3
003			DE-He213
005			20140220084505.0
007			cr nn 008mamaa
008			100301s2010 xxu| s |||| 0|eng d
020			_a9781441913043 _9978-1-4419-1304-3
024	7		_a10.1007/978-1-4419-1304-3 _2doi
050		4	_aQH345
050		4	_aQD415-436
072		7	_aPSB _2bicssc
072		7	_aSCI007000 _2bisacsh
082	0	4	_a572 _223
100	1		_aBurry, Richard W. _eauthor.
245	1	0	_aImmunocytochemistry _h[electronic resource] : _bA Practical Guide for Biomedical Research / _cby Richard W. Burry.
264		1	_aNew York, NY : _bSpringer New York, _c2010.
300			_aXV, 209p. 82 illus., 8 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aAntibodies -- Sample Preparation/Fixation -- Tissue Sectioning -- Blocking and Permeability -- Labels for Antibodies -- Application Methods -- Controls -- Method and Label Decision -- Single Antibody Procedure -- Multiple Antibodies Different Species -- Multiple Antibodies from the Same Species -- Fluorescent Microscopy and Imaging -- Troubleshooting -- Electron Microscopic Immunocytochemistry.
520			_aImmunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book’s predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.
650		0	_aLife sciences.
650		0	_aImmunology.
650		0	_aBiochemistry.
650		0	_aCytology.
650		0	_aMicroscopy.
650	1	4	_aLife Sciences.
650	2	4	_aBiochemistry, general.
650	2	4	_aLife Sciences, general.
650	2	4	_aAnimal Biochemistry.
650	2	4	_aBiological Microscopy.
650	2	4	_aImmunology.
650	2	4	_aCell Biology.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781441913036
856	4	0	_uhttp://dx.doi.org/10.1007/978-1-4419-1304-3
912			_aZDB-2-SBL
999			_c110367 _d110367