000 03157nam a22004335i 4500
001 978-1-4419-1234-3
003 DE-He213
005 20140220084505.0
007 cr nn 008mamaa
008 100715s2010 xxu| s |||| 0|eng d
020 _a9781441912343
_9978-1-4419-1234-3
024 7 _a10.1007/978-1-4419-1234-3
_2doi
050 4 _aRB1-214
072 7 _aMMF
_2bicssc
072 7 _aMED067000
_2bisacsh
082 0 4 _a616.07
_223
100 1 _aPeters, Stephen R.
_eeditor.
245 1 2 _aA Practical Guide to Frozen Section Technique
_h[electronic resource] /
_cedited by Stephen R. Peters.
264 1 _aNew York, NY :
_bSpringer New York,
_c2010.
300 _aXII, 194p. 98 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aUnderstanding and Maintaining the Cryostat -- Gross Examination of Tissues in the Frozen Section Room -- Embedding of Tissue for Frozen Section -- Cutting the Frozen Section -- Variables Affecting the Cutting Properties of Tissues and the Resulting Artifacts -- Fixation, Staining and Coverslipping of Frozen Section Slides -- Microscopic Interpretation in the Frozen Section Setting -- Frozen Section Techniques Used in Mohs Micrographic Surgery -- Frozen Section Technique in the Animal Research Setting.
520 _aA Practical Guide to Frozen Section Technique offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step by step approach to learning continuous motion brush technique. Emphasis is placed on recognizing and correcting artifacts during the preparation process. The book addresses all of the steps in the preparation of slides from cutting through cover-slipping. The author’s unique, original techniques for tissue embedding including face down embedding in steel well bars, frozen block cryoembedding and paper cryoembedding are detailed. Variables key to the quality of the preparation including block temperature, tissue properties and section thickness are detailed. The book also covers understanding the cryostat and basic maintenance and care.Sections covering techniques used in Mohs dermatologic surgery, and techniques used in basic animal and human research are discussed by noted experts in their field. A Practical Guide to Frozen Section Technique will be of great value to pathologists, pathology residents in training and also experimental pathology researchers that rely upon this methodology to perform tissue analysis in research.
650 0 _aMedicine.
650 0 _aMedical laboratories.
650 0 _aPathology.
650 1 4 _aMedicine & Public Health.
650 2 4 _aPathology.
650 2 4 _aLaboratory Medicine.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781441912336
856 4 0 _uhttp://dx.doi.org/10.1007/978-1-4419-1234-3
912 _aZDB-2-SME
999 _c110350
_d110350