000 03439nam a22004455i 4500
001 978-3-642-17890-0
003 DE-He213
005 20140220083752.0
007 cr nn 008mamaa
008 110606s2011 gw | s |||| 0|eng d
020 _a9783642178900
_9978-3-642-17890-0
024 7 _a10.1007/978-3-642-17890-0
_2doi
050 4 _aRB1-214
072 7 _aMMF
_2bicssc
072 7 _aMED067000
_2bisacsh
082 0 4 _a616.07
_223
100 1 _aStanta, Giorgio.
_eeditor.
245 1 0 _aGuidelines for Molecular Analysis in Archive Tissues
_h[electronic resource] /
_cedited by Giorgio Stanta.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg,
_c2011.
300 _aXVI, 323p. 46 illus., 31 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aIntroduction -- Archive Tissues -- Pre-Analytical Treatment of Tissues -- Dissection of Tissue Components -- Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids -- Reverse Transcription (RT) -- Nucleic Acid Amplification -- DNA Sequencing -- Microsatellite Analysis -- Methylation Analysis -- Analysis of Copy Number Variations -- Microarrays -- Protein Extraction in Archive Tissues -- Protein Electrophoretic Separation -- Protein Immunodetection -- Mass Spectrometry -- Elements of Good Laboratory Practice. -- DNA Sequencing -- Microsatellite Analysis -- Methylation Analysis -- Analysis of Copy Number Variations -- Microarrays -- Protein Extraction in Archive Tissues -- Protein Electrophoretic Separation -- Protein Immunodetection -- Mass Spectrometry -- Elements of Good Laboratory Practice.
520 _aA huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care.   This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.
650 0 _aMedicine.
650 0 _aOncology.
650 0 _aPathology.
650 1 4 _aMedicine & Public Health.
650 2 4 _aPathology.
650 2 4 _aMolecular Medicine.
650 2 4 _aOncology.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783642178894
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-642-17890-0
912 _aZDB-2-SME
999 _c107322
_d107322