000 02925nam a22004815i 4500
001 978-3-642-10729-0
003 DE-He213
005 20140220083741.0
007 cr nn 008mamaa
008 110103s2011 gw | s |||| 0|eng d
020 _a9783642107290
_9978-3-642-10729-0
024 7 _a10.1007/978-3-642-10729-0
_2doi
050 4 _aQD71-142
072 7 _aPNFC
_2bicssc
072 7 _aSCI013010
_2bisacsh
082 0 4 _a543.8
_223
100 1 _aSpangenberg, Bernd.
_eauthor.
245 1 0 _aQuantitative Thin-Layer Chromatography
_h[electronic resource] :
_bA Practical Survey /
_cby Bernd Spangenberg, Colin F. Poole, Christel Weins.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2011.
300 _aXV, 388p. 126 illus., 72 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aHistory of Planar Chromatography -- The Theoretical Basis of Thin-Layer Chromatography (TLC) -- The Stationary Phase in Thin Layer Chromatography (TLC) -- The Mobile Phase in Adsorption and Partition Chromatography -- Preparing and Applying Samples -- Basis for TLC Development Techniques -- Specific Staining Reactions -- Bioeffective-linked Analysis in Modern HPTLC -- Planar Chromatography Detectors -- Diffuse Reflectance from TLC Layers -- Fluorescence in TLC Layers -- Chemometrics in HPTLC -- Reciprocal Model -- Statistics for Quantitative TLC -- Planning an Analysis and Validation in TLC.
520 _aThin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.
650 0 _aChemistry.
650 0 _aChromatography.
650 0 _aFood science.
650 0 _aPharmacy.
650 1 4 _aChemistry.
650 2 4 _aChromatography.
650 2 4 _aPharmacy.
650 2 4 _aFood Science.
700 1 _aPoole, Colin F.
_eauthor.
700 1 _aWeins, Christel.
_eauthor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783642107276
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-642-10729-0
912 _aZDB-2-CMS
999 _c106733
_d106733