000 03994nam a22005055i 4500
001 978-1-4419-7251-4
003 DE-He213
005 20140220083723.0
007 cr nn 008mamaa
008 101118s2011 xxu| s |||| 0|eng d
020 _a9781441972514
_9978-1-4419-7251-4
024 7 _a10.1007/978-1-4419-7251-4
_2doi
050 4 _aQH345
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
082 0 4 _a572
_223
100 1 _aBuxbaum, Engelbert.
_eauthor.
245 1 0 _aBiophysical Chemistry of Proteins
_h[electronic resource] :
_bAn Introduction to Laboratory Methods /
_cby Engelbert Buxbaum.
264 1 _aBoston, MA :
_bSpringer US :
_bImprint: Springer,
_c2011.
300 _aXVI, 510p. 168 illus., 116 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aAnalytical techniques. Microscopy. Single molecule techniques. Preparation of cells and tissues for microscopy. Principles of optical spectroscopy.Photometry. Fluorimetry. Chemiluminescence.Electrophoresis.Immunological methods.Isotope techniques -- Purification of proteins.Homogenisation and fractionisation of cells and tissues.Isolation of organelles. Precipitation methods.Chromatography. Membrane proteins. Determination of protein concentration.Cell culture -- Protein modification and inactivation. General technical remarks. Amine-reactive reagents. Thiol- and disulphide reactive reagents. Reagents for other groups. Cross-linkers.Detection methods. Spontaneous reactions in proteins -- Protein size and shape. Centrifugation.Osmotic pressure. Diffusion.Viscosity.Non-resonant interactions with electromagnetic waves -- Protein structure. Protein sequencing.Synthesis of peptides. Protein secondary structure. Structure of protein-ligand complexes. 3D-structures. Folding and unfolding of proteins -- Enzyme kinetics. Steady-state kinetics. Leaving the steady state: Analysis of progress curves.Reaction velocities. Isotope effects. Isotope exchange -- Protein-ligand interactions. General conditions for interpretable results. Binding equations.Methods to measure binding equilibria. Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology. Industrial enzyme use.Immobilised enzymes -- Special statistics.Quality control. Testing whether or not a model fits the data -- Appendix. List of symbols.Greek alphabet. Properties of electrophoretic buffers. Bond properties. Acronyms.
520 _aUndergraduate biochemistry courses cover what proteins do, as enzymes, receptors, hormones, motors or structural components. The much more interesting question is how can proteins achieve all these functions? Presented here is an overview of the methods used in such projects, their possible applications, and their limitations. Focusing on the biophysical chemistry of proteins, the text is accessible to those with a general background in chemistry, physics and mathematics, though a good understanding of protein structure and enzymology is required. The text may be used in courses of protein science, by students embarking on master- or PhD-thesis work in this area or by professionals who need a quick reminder about the essentials of a method.
650 0 _aLife sciences.
650 0 _aMedicine.
650 0 _aChemistry.
650 0 _aBiochemistry.
650 0 _aCytology.
650 1 4 _aLife Sciences.
650 2 4 _aBiochemistry, general.
650 2 4 _aCell Biology.
650 2 4 _aChemistry/Food Science, general.
650 2 4 _aBiomedicine general.
650 2 4 _aBiophysics and Biological Physics.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781441972507
856 4 0 _uhttp://dx.doi.org/10.1007/978-1-4419-7251-4
912 _aZDB-2-SBL
999 _c105720
_d105720