000 04124nam a22004815i 4500
001 978-3-7091-0154-4
003 DE-He213
005 20140220083331.0
007 cr nn 008mamaa
008 111202s2012 au | s |||| 0|eng d
020 _a9783709101544
_9978-3-7091-0154-4
024 7 _a10.1007/978-3-7091-0154-4
_2doi
050 4 _aQD71-142
072 7 _aPNF
_2bicssc
072 7 _aSCI013010
_2bisacsh
082 0 4 _a543
_223
100 1 _aWilliams, S. Kim R.
_eeditor.
245 1 0 _aField-Flow Fractionation in Biopolymer Analysis
_h[electronic resource] /
_cedited by S. Kim R. Williams, Karin D. Caldwell.
264 1 _aVienna :
_bSpringer Vienna,
_c2012.
300 _aX, 305p. 93 illus., 44 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aIntroduction -- 1 Flow FFF - Basics and Key Applications -- 2 Assessing protein-ultrafiltration membrane interactions using flow field-flow fractionation -- 3 Hollow-fiber flow field-flow fractionation: a pipeline to scale down separation and enhance detection of proteins and cells -- 4 Two-Dimensional Separation for Proteomic Analysis -- 5 Field-Flow Fractionation in Therapeutic Protein Development -- 6 Assessing and Improving Asymmetric Flow Field Flow Fractionation of Therapeutic Proteins -- 7 Studies of loose protein aggregates by flow Field-Flow Fractionation (FFF) coupled to Multi-Angle Laser Light Scattering (MALLS) -- 8 Field Flow Fractionation for Assessing Biomolecular Interactions in Solution -- 9 Flow Field Flow Fractionation: Analysis of Biomolecules and Their Complexes -- 10 Analysis of prions by field-flow fractionation -- 11 Multifunctionalized Particles for Biosensor Use -- 12 Starch and other polysaccharides -- 13 The use of field-flow fractionation for the analysis of drug and gene delivery systems -- 14 Characterization of liposomes by FFF -- 15 Mammalian Cell Sorting with Sedimentation Field Flow Fractionation -- 16 Isolation and characterization of cells by Dielectrophoretic Field-Flow Fractionation -- 17 Field flow fractionation coupled to inductively coupled plasma-mass spectrometry (FFF-ICP-MS): Methodology and application to environmental nanoparticle research.  .
520 _aThis is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.    
650 0 _aChemistry.
650 0 _aPharmaceutical technology.
650 0 _aAnalytical biochemistry.
650 0 _aBiochemistry.
650 1 4 _aChemistry.
650 2 4 _aAnalytical Chemistry.
650 2 4 _aPharmaceutical Sciences/Technology.
650 2 4 _aProtein Science.
650 2 4 _aMedicinal Chemistry.
700 1 _aCaldwell, Karin D.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783709101537
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-7091-0154-4
912 _aZDB-2-CMS
999 _c103899
_d103899