000 02916nam a22005175i 4500
001 978-1-4419-7808-0
003 DE-He213
005 20140220083233.0
007 cr nn 008mamaa
008 110927s2012 xxu| s |||| 0|eng d
020 _a9781441978080
_9978-1-4419-7808-0
024 7 _a10.1007/978-1-4419-7808-0
_2doi
050 4 _aQD241-441
072 7 _aPSB
_2bicssc
072 7 _aSCI013040
_2bisacsh
072 7 _aSCI007000
_2bisacsh
082 0 4 _a547
_223
100 1 _aZhang, Xin.
_eauthor.
245 1 0 _aMultistate GTPase Control Co-translational Protein Targeting
_h[electronic resource] /
_cby Xin Zhang.
264 1 _aBoston, MA :
_bSpringer US,
_c2012.
300 _aVII, 96p. 46 illus., 31 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Theses
505 0 _aIntroduction -- A Multistep Mechanism for Assembly of the SRP-SR Complex -- Dynamics of the Transient Intermediate during SRP-SR Association -- Multiple Conformational Switches Control Cotranslational Protein Targeting -- Sequential Checkpoints Govern Substrate Selection during Cotranslational Protein Targeting.
520 _aProteins act as macromolecular machinery that mediate many diverse biological processes - the molecular mechanisms of this machinery has fascinated biologists for decades. Analysis of the kinetic and thermodynamic features of these mechanisms could reveal unprecedented aspects of how the machinery function and will eventually lead to a novel understanding of various biological processes. This dissertation comprehensively demonstrates how two universally conserved guanosine triphosphatases in the signal recognition particle and its membrane receptor maintain the efficiency and fidelity of the co-translational protein targeting process essential to all cells. A series of quantitative experiments reveal that the highly ordered and coordinated conformational states of the machinery are the key to their regulatory function. This dissertation also offers a mechanistic view of another fascinating system in which multistate protein machinery closely control critical biological processes.
650 0 _aChemistry.
650 0 _aAnalytical biochemistry.
650 0 _aBioorganic chemistry.
650 0 _aBiochemistry.
650 0 _aProteomics.
650 1 4 _aChemistry.
650 2 4 _aBioorganic Chemistry.
650 2 4 _aProteomics.
650 2 4 _aAnalytical Chemistry.
650 2 4 _aBiochemistry, general.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781441978073
830 0 _aSpringer Theses
856 4 0 _uhttp://dx.doi.org/10.1007/978-1-4419-7808-0
912 _aZDB-2-CMS
999 _c100544
_d100544